AUDIT READOUT AND VALIDATION RESULTS · private client work; enzymelens

WEEK 30 · Mon 12 Apr → Sun 18 Apr 2027

Audit readout and validation results

600 minutes max9 deliverables private client work; enzymelens
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UNDERSTAND · PRACTICE · KEEP EVIDENCE

Your full learning notebook

Read the week in plain language, refresh the foundations, follow each challenge step by step, and check what evidence to keep. Includes worked examples, answer keys and prompts for visual study resources.

Use this within your existing weekly blocks. Your learning completion and research evidence remain in your current tracker and week files.

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START HERE · 2 MINUTES

What am I doing right now?

If you only complete one thing:
✓

STATUS

Tracker & deliverables

DeliverableAcceptanceState
audit-final-readout.md
Build create +
Complete the audit's three prioritized automation candidates with measured baseline, estimated days saved, uncertainty, cost assumptions and owner. Acceptance: Complete the contracted audit readout or documented delivery recovery, and preserve any received partner data with provenance.
paper-3-validation-results.md
Build create +
Analyze received wet-lab data against the preregistered plan with controls and biological replicate counts; use a computational-only analysis if no data arrived. Acceptance: Observed versus predicted outcomes and deviations are separate.
case-study-draft.md
Build create +
Prepare the anonymized case-study draft and Paper 3 figures; audit residual delivery work and author review dates. Acceptance: Client claims trace to evidence; publication permission is requested separately.
study-notes.md
Learning create +
Selected exact free resources; bounded lesson/exercise; separate solution; quiz and grading rubric; Peter's answers and gaps when executed
founder-paper.md
Founder papers create +
Verified source and P11 digest; authentic question and connection sentence; access limitations
branding-pack.md
Branding create +
Wednesday note, Sunday build log, X adaptation, figure/caption/alt text and the week's special asset; each claim linked to real proof
opportunity-actions.md
Opportunities create +
Qualified target/role, source/date, ladder stage, next action, message/interview/proposal draft and actual status
evidence.md
Evidence & review open & edit ↗
Actual paths/URLs, versions, checks/metrics, preparation versus execution state and external receipts when applicable
review.md
Evidence & review open & edit ↗
Checklist, learning assessment, gate status, shortfalls, next actions and carryover
Notes on artifact states

These filenames are outputs to prepare during this week's execution; this scaffold does not claim they already exist. Drafts and measured results have different states. Technical code/data stay in the canonical project repository and are referenced from these coordinating files.

During preparation, future measured tables get column definitions, input requirements and run instructions with PENDING status. During execution, replace pending values only with measured outputs. Do not create realistic-looking fake evidence.

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600 MINUTES MAXIMUM

Seven-day plan

Monday90 min

Target. Complete the audit's three prioritized automation candidates with measured baseline, estimated days saved, uncertainty, cost assumptions and owner. Stop when: Complete the contracted audit readout or documented delivery recovery, and preserve any received partner data with provenance.

No material for Monday — this is a pure build day. Need something to study instead? →

Tuesday90 min· 3 cards, 60 min

Target. Revisit MSMB/ISLP bootstrap and small-sample inference; define the actual biological unit and descriptive limits of partner assays. Produce: One concise applied note, a worked decision/example and unresolved questions.

Do these in this order — the plan's own sequence:

  1. Read Ho et al. (2019), "Moving beyond P values: data analysis with estimation graphics" 15 min find ↗
  2. Read Cumming (2014) "The new statistics" 30 min · after Ho et al. (2019), "Moving beyond P values: data analysis with estimation graphics" find ↗
  3. Watch StatQuest "Statistical Power" 15 min? · after Cumming (2014) "The new statistics" find ↗
Wednesday90 min

Target. Analyze received wet-lab data against the preregistered plan with controls and biological replicate counts; use a computational-only analysis if no data arrived. Stop when: Observed versus predicted outcomes and deviations are separate. Reserve the last 10 minutes for the optional Wednesday note.

No material for Wednesday — this is a pure build day. Need something to study instead? →

Thursday90 min

Target. Apply the preregistered analysis to received data and reconcile audit delivery evidence; no new course. Relevant partner paper within remaining time. Apply the Half 2 one-formal-study-block rule.

No material for Thursday — this is a pure build day. Need something to study instead? →

Friday90 min

Target. Prepare the anonymized case-study draft and Paper 3 figures; audit residual delivery work and author review dates. Stop when: Client claims trace to evidence; publication permission is requested separately.

No material for Friday — this is a pure build day. Need something to study instead? →

Saturday90 min

Target. Resolve the earliest critical spillover or dependency. Prepare any long-form publication draft from existing figures here if needed. Sandbox only after commitments are met.

Optional, only if the day went well:

  1. Run Vaux, Fidler & Cumming (2012), "Replicates and repeats—what is the difference and is it signific… Saturday sandbox · 30 min? find ↗
  2. Read Coursera Introduction to Statistics Saturday sandbox · 30 min? · after Vaux, Fidler & Cumming (2012), "Replicates and repeats—what is the difference and is it signific… find ↗
Sunday60 min

Target. 20 min build-log draft/publication when authorized; 5 min evidence/checklist; 20 min source-grounded gap review; 15 min next week's six physical actions.

No material for Sunday — this is a pure build day. Need something to study instead? →

Any time this week5 cards, 140 min · the plan names no day for these

Spare pockets, repo exercises and anything the plan lists without a day. Take them in this order when a day finishes early.

  1. Read Weissgerber et al. (2015), "Beyond bar and line graphs: time for a new data presentation paradigm" 20 min find ↗
  2. Run ACCLAB/DABEST-python 30 min open ↗
  3. Run mwaskom/seaborn 30 min open ↗
  4. Run arviz-devs/arviz 30 min open ↗
  5. Run lmfit/lmfit-py 30 min open ↗
Block ritual: read your next action → start the 90-min timer → open one file. Close: write tomorrow's action and one log line.
Feels pointless? Does today's Done when need it? No → skip it and log it. Yes → let AI do the boring part, you review. Still stuck → the smallest version in 25 minutes. Pulled into a rabbit hole? Write it in Parked thoughts and save it for Saturday.

The model must turn these into exact physical actions using the observed files and available inputs. Each 90-minute block includes start/stop rituals. All calls, posts, debugging, reading and exercises consume this same capacity.

Half 2: Tuesday is the formal study block unless this is a selling/submission/decision week. Thursday supports applied review, partner work or the market task; do not add another full course.

◎

WHAT TO WATCH, READ AND RUN

Materials for this week

Two deliveries meet this week, and they rhyme. For the client, you close the audit with a readout and an anonymised case study (with permission). For the partner, you open the envelope: the wet-lab results for your candidates, analysed exactly as pre-registered in week 24. Small numbers of samples need special care: with three replicates, one outlier can create or destroy a "finding". Think of a restaurant with thre…

0 of 10 finished · give me one to start now → · open in the deck →

In the plan's running order: each card says which block it belongs to and what has to come before it. The seven-day plan lists the same cards under the day that owns them.

Tuesday 3 · 60 min total

1/10 Read 15 min W30 Tuesday Biology

Ho et al. (2019), "Moving beyond P values: data analysis with estimation graphics"

Ho et al. (2019), "Moving beyond P values: data analysis with estimation graphics", Nature Methods 16:565-566 (15 min)

Find it ↗ search

In: LABLOOP-W30 Notebook

2/10 Read 30 min W30 Tuesday Biology

Cumming (2014) "The new statistics"

Tuesday · after Ho et al. (2019), "Moving beyond P values: data analysis with estimation graphics"

Cumming (2014) "The new statistics" (carry over), sections on small samples (30 min)

Find it ↗ search

In: LABLOOP-W30 Notebook

3/10 Watch 15 min? W30 Tuesday Biology

StatQuest "Statistical Power"

Tuesday · after Cumming (2014) "The new statistics"

StatQuest "Statistical Power" (carry over)

Find it ↗ search

In: LABLOOP-W30 Notebook

Saturday · sandbox, optional 2 · 60 min total

4/10 Run 30 min? W30 Saturday Sandbox optional

Vaux, Fidler & Cumming (2012), "Replicates and repeats—what is the difference and is it signific…

Saturday sandbox

Vaux, Fidler & Cumming (2012), "Replicates and repeats—what is the difference and is it significant?", EMBO Reports 13:291-296

Find it ↗ search

In: LABLOOP-W30 Notebook

5/10 Read 30 min? W30 Saturday Sandbox optional

Coursera Introduction to Statistics

Saturday sandbox · after Vaux, Fidler & Cumming (2012), "Replicates and repeats—what is the difference and is it signific…

Coursera Introduction to Statistics (Stanford), module on small-sample inference

Find it ↗ search

In: LABLOOP-W30 Notebook

Any block this week — the plan names no day 5 · 140 min total

6/10 Read 20 min W30 Craft

Weissgerber et al. (2015), "Beyond bar and line graphs: time for a new data presentation paradigm"

Weissgerber et al. (2015), "Beyond bar and line graphs: time for a new data presentation paradigm", PLoS Biology 13(4):e1002128 (20 min)

Find it ↗ search

In: LABLOOP-W30 Notebook

7/10 Run 30 min W30 Build

ACCLAB/DABEST-python

Estimation plots (Gardner-Altman) for measured activity vs control; the library from Ho et al. 2019

Open ↗

In: LABLOOP-W30 Notebook

8/10 Run 30 min W30 Build

mwaskom/seaborn

Strip plots with every replicate over the predicted ranking

Open ↗

In: LABLOOP-W30 Notebook

9/10 Run 30 min W30 Build

arviz-devs/arviz

If you fit a small Bayesian model, plot posteriors per candidate

Open ↗

In: LABLOOP-W30 Notebook

10/10 Run 30 min W30 Build

lmfit/lmfit-py

Run the frozen week 24 analysis script on the real data, unchanged

Open ↗

In: LABLOOP-W30 Notebook

01

READ FIRST

Outcome and relevance

This week delivers the three concrete artifacts below and the supporting learning/branding/opportunity pack. The scientific or commercial outcome follows the preserved source brief, while the acceptance checks make its evidence testable. The career objective is to turn this work into something a scientist, hiring manager or buyer can inspect.

  • Scientific/build priority: Complete the audit's three prioritized automation candidates with measured baseline, estimated days saved, uncertainty, cost assumptions and owner.
  • Public/profile priority: Draft an audit lesson and validation figure summary; client-specific publication remains contingent on permission.
  • Career/relationship/commercial priority: Prepare a second-audit or pilot next step from actual findings; ask for publishable feedback only after delivery.
02

READ FIRST

Deeper walkthrough (v6)

Read v6 · Week 30 · Audit delivery 2; Paper 3 results before Monday. It expands this week's one-row v5 brief into daily build steps and adds the Welcome analogy, Learn it (▶ core and + deeper sources), Run it (repos with a 20-40 minute exercise), the NotebookLM kit and the mentor check below. Standing v6 rules: resource rules, NotebookLM protocol, chapter anatomy. v6 adds depth only; it does not change a goal, gate, output or the 600-minute cap, so this specification still governs execution.

Act VI · Threads: M, $, EL · Next gate: 32

Where this week sits. - ← From: week 24 pre-registration, week 28 raw results, week 29 audit. - → Feeds: week 31 (Paper 3 draft), week 32 (preprint + case study), week 38 (talk), week 39 (fund package uses the case study). - ⟳ Returns in: L4 co-authored and the commercial proof of Act VI.

Goals. - 🎯 This week: audit final readout; anonymised case study drafted; partner validation analysed per plan; Paper 3 figures. - 🏁 Next milestone: Gate 32. - 🌟 Final: Paper 3 with real validation and a public case study.

The plan (from v5 Part 7, expanded; gate and output unchanged).

v5 row: 30 | Audit delivery 2; Paper 3 results | Statistics for validation results (small n) | Analysis of the partner's validation; figures | Anonymized case study drafted - Goal: two honest reports: one for the client, one for science. - Tue (study): statistics for small n: estimation plots, bootstrap limits with n = 3, why to show every point. - Build: Mon: audit readout delivered; three candidates with days saved; client feedback captured; case-study permission asked. Wed: validation analysis exactly as in PREREGISTRATION.md; deviations from the plan listed explicitly. Done when every pre-registered analysis has a result. Fri: Paper 3 figures (predicted vs measured, per candidate, all replicates shown); case study draft (anonymised). - Sun: L-Sun only with permissions; otherwise "what a pre-registration felt like when the data arrived". - Only one thing: the pre-registered analysis run.

Mentor check. "I can show whether my predictions held in a real lab, with every replicate visible, and I delivered a client readout the same week."

03

OPEN WHEN NEEDED

Required inputs and dependency check

04

READ FIRST

Learning specification

Biology/domain unit

Material: Revisit MSMB/ISLP bootstrap and small-sample inference; define the actual biological unit and descriptive limits of partner assays.

Produce: One concise applied note, a worked decision/example and unresolved questions.

Understanding check: The note directly supports this week's experiment, collaboration, delivery or career action.

During preparation, write one worked example connected to the week's actual task, one exercise with a separate answer key and five questions. Peter supplies answers; score explanation quality and flag misconceptions in QUESTIONS. Numeric/scientific conclusions require actual data or explicitly synthetic teaching values.

AI/scientific-method unit

Material: Apply the preregistered analysis to received data and reconcile audit delivery evidence; no new course. Relevant partner paper within remaining time.

Produce: One concise applied note, a worked decision/example and unresolved questions.

Understanding check: The note directly supports this week's experiment, collaboration, delivery or career action.

Use the prompt pack: P1 explanation ladder, P2 concept-to-project bridge and P3 quiz. Coding practice belongs within the allocated study/build block. Where the source asks for a favorable scientific result, substitute an honest measured comparison as explained below.

Founder/scientist paper

Select one verified founder/scientific-lead paper relevant to this week's domain. Use P11; save the question, method, numerical result with context, data workflow, three author questions and one connection sentence in founder-paper.md. Keep abstract-only access limitations explicit. On source weeks with no study, prioritize the commercial/submission task and log any skipped digest.

05

OPEN WHEN NEEDED

Branding and opportunity execution brief

Branding: Draft an audit lesson and validation figure summary; client-specific publication remains contingent on permission.

Opportunity action: Prepare a second-audit or pilot next step from actual findings; ask for publishable feedback only after delivery.

Use career asset contracts. Publish at most the agreed cadence; one figure and one supported number or admitted failure can support multiple drafts. In long-form weeks, prepare a 1,500–2,500-word article from existing evidence within the build/buffer allocation. In release/demo weeks, prepare a 60-second script: problem, input, command/action, result, limitation and proof link.

Verify individuals, jobs and affiliations at execution time. The GridX list and v4 company map are research seeds, not current facts. Do not reveal named company weaknesses or employer/client confidential information. Keep drafted/sent/replied/booked/held statuses separate. The planning request alone does not authorize external messages or publication; honor explicit authorization in the execution session.

06

OPEN WHEN NEEDED

Quality risks and week-specific constraints

Do not force wet-lab results to arrive on schedule. Two major workstreams compete for capacity; prioritize contracted obligations and record research slippage rather than inventing closure.

Apply the root scientific evidence contract: provenance; meaningful independent splits; no future-data leakage; baseline comparison; actual denominators/uncertainty; failed cases; human review where required. State whether data are simulated, public experimental, synthetic or partner-provided.

Keep source gates intact, and log scope/time conflicts in review. Missing people, money, access or results must not be replaced with invented evidence. Reduce optional polish first. A specific essential external dependency stays pending while independent preparation continues.

07

READ FIRST

Completion and gate review

This is an enabling week. Its deliverables feed the next scheduled gate: 32. Complete the acceptance checklist below; do not promote reputation from a draft.

  • Monday artifact satisfies: Complete the contracted audit readout or documented delivery recovery, and preserve any received partner data with provenance.
  • Wednesday artifact satisfies: Observed versus predicted outcomes and deviations are separate.
  • Friday artifact satisfies: Client claims trace to evidence; publication permission is requested separately.
  • Study pack is prepared; Peter's actual learning/quiz result is recorded separately.
  • Branding and opportunity drafts use supported claims and the correct external-action state.
  • Reproduction or document checks are recorded with file/version references.
  • This week's special gate, if any, has real evidence or remains explicitly pending.
  • Review, root progress/log and the next physical action are updated.
08

READ FIRST

Minimum viable week and recovery

Expanded minimum derived from the source goal: Complete the contracted audit readout or documented delivery recovery, and preserve any received partner data with provenance.

Operational first step if stalled: open audit-final-readout.md, write the required inputs and produce the smallest checkable example in ten minutes. If a more urgent missing gate dependency controls the week, use the source minimum above.

If the full week does not fit, use P14: retain one gate-critical deliverable and one directly supporting study exercise; record what is skipped versus moved. Do not mark the original full gate passed merely because the minimum was completed. After a missed week, reforecast from actual evidence and avoid a second unexamined lost week.

09

OPEN WHEN NEEDED

Handoff to the next model

In review, state exactly what exists, what was verified, what Peter can now explain, which external events remain pending, where the canonical code/data live, and the next file/function or draft paragraph to touch. Link each fact to evidence. Carry scientific assumptions, split definitions and rights constraints forward explicitly.

10

OPEN WHEN NEEDED

Ready-to-copy model prompt

Prepare Week 30 of this LABLOOP execution plan.
Read execution-plan/specs.md, execution-plan/DECISIONS.md,
execution-plan/GATES.md and
execution-plan/weeks/week-30-audit-readout-and-validation-results/specs.md, commitment.md, evidence.md and review.md.
Read prior prerequisite reviews and inspect actual repository/data state.
Use v5 as the governing sequence, the v6 Week 30 chapter
(LABLOOP-Master-Plan-v6.md) as the deeper walkthrough, and v4 only for
retained details.
Produce the full local preparation pack and the exact week-specific files named
in the artifact table. Give me a bounded lesson with exercises/answer key,
daily physical actions, technical runbook or draft, branding drafts and
career/commercial actions. Keep the total to 600 minutes.
Separate prepared artifacts from measured results and my learning completion.
Verify sources and do not invent metrics, citations, papers, people or outcomes.
Do not execute external communications, publication or spending unless the
current session already authorizes the specific action.
Finish by updating evidence/review and TODAY with the next physical action.
11

OPEN WHEN NEEDED

Preserved original v5 weekly brief

v6 expands this row into a full brief, with the gate and output unchanged, inside v6 · Week 30 · Audit delivery 2; Paper 3 results. The following is source planning text, not a claim of completion. Apply the explicit evidence corrections in this specification and DECISIONS.md.

Week Goal Study (Tue) Build (Mon/Wed/Fri) Output
30 Audit delivery 2; Paper 3 results Statistics for validation results (small n) Analysis of the partner's validation; figures Anonymized case study drafted
📄

WEEK FILES

Commitment · evidence · review

commitment.md open ↗

State: UNCONFIRMED. Owner: Peter Ciancaglini. Specification

Start date: not set. End date: not set. Available hours: confirm (planned ceiling 10). Primary outcome: audit readout and validation results. Minimum that keeps the next gate alive: Complete the contracted audit readout or documented delivery recovery, and preserve any received partner data with provenance.

Deliverables to commit

  • ☐ audit-final-readout.md: Complete the contracted audit readout or documented delivery recovery, and preserve any received partner data with provenance.
  • ☐ paper-3-validation-results.md: Observed versus predicted outcomes and deviations are separate.
  • ☐ case-study-draft.md: Client claims trace to evidence; publication permission is requested separately.
  • ☐ Bounded learning exercise and personal teach-back.
  • ☐ Evidence-backed branding and opportunity pack.
  • ☐ Sunday review and next physical action.

Proposed calendar

Day Minutes Physical action / outcome Actual slot Done
Mon 90 Complete the audit's three prioritized automation candidates with measured baseline, estimated days saved, uncertainty, cost assumptions and owner. Not set ☐
Tue 90 One concise applied note, a worked decision/example and unresolved questions. Not set ☐
Wed 90 Analyze received wet-lab data against the preregistered plan with controls and biological replicate counts; use a computational-only analysis if no data arrived. Last ten minutes: optional note. Not set ☐
Thu 90 One concise applied note, a worked decision/example and unresolved questions. Include relevant founder-paper work within this block. Not set ☐
Fri 90 Prepare the anonymized case-study draft and Paper 3 figures; audit residual delivery work and author review dates. Not set ☐
Sat 90 Resolve critical spillover; long-form draft if due; sandbox only if done. Not set ☐
Sun 60 Build log, evidence, gap review, next actions. Not set ☐

Dependency and capacity check

  • Missing essential inputs: not yet inspected.
  • Human/external dependency, owner and expected date: not yet inspected.
  • Week-specific constraint: Do not force wet-lab results to arrive on schedule. Two major workstreams compete for capacity; prioritize contracted obligations and record research slippage rather than inventing closure.
  • Existing session authorization for external actions or spending: not assessed; local planning only at scaffold creation.
  • Scope to cut first: optional polish/additional examples; preserve scientific validity.
  • If the forecast exceeds available time: record a concrete reduced scope or revised delivery proposal before committing.

Peter's commitment / adjustments: pending. Reviewing model and date: pending.

evidence.md open ↗

Execution state: NOT_STARTED. Specification This file is a blank register with planned artifact names; none of the rows prove completion.

Item Path / URL / version State Verification / actual result Verified by / date
audit-final-readout.md Not created NOT_STARTED Pending: Client acceptance and any remaining prototype limits are recorded. —
paper-3-validation-results.md Not created NOT_STARTED Pending: Observed versus predicted outcomes and deviations are separate. —
case-study-draft.md Not created NOT_STARTED Pending: Client claims trace to evidence; publication permission is requested separately. —
Learning exercise / Peter's quiz Not created NOT_STARTED Peter's actual answers required —
Founder paper digest Not created NOT_STARTED Source/content and access checked —
Branding pack Not created NOT_STARTED Claims audited; drafted/public status distinct —
Opportunity actions Not created NOT_STARTED Drafted/sent/replied/booked/held distinct —

Reproduction and scientific measurements

Question / metric Dataset, N and unit Split / seed / config Command or verification method Output path and measured value Uncertainty / limitation

External events, when authorized and actually performed

Date Action / counterpart Receipt or private reference State Next action / date

Actual learning and decisions

Peter's teach-back / quiz score: not assessed. Scientific assumptions and deviations: none recorded yet. Budget allocated / spent: not assessed / no spend recorded. Missing evidence and responsible owner: pending inspection.

Do not put API keys, source full texts with restricted rights, client data or invoice personal details in a public version of this register. Keep private evidence references private.

review.md open ↗

Review status: NOT_REVIEWED. Specification · Evidence

What was actually completed?

  • ☐ Complete the contracted audit readout or documented delivery recovery, and preserve any received partner data with provenance. Evidence: pending.
  • ☐ Observed versus predicted outcomes and deviations are separate. Evidence: pending.
  • ☐ Client claims trace to evidence; publication permission is requested separately. Evidence: pending.
  • ☐ Learning assessed from Peter's actual answers. Evidence: pending.
  • ☐ Branding/opportunity states recorded correctly. Evidence: pending.

Learning and outcome

What Peter can explain or reproduce now: not assessed. Quiz / teach-back gaps: not assessed. Measured result and limitations: pending. Best evidence link for a scientist/recruiter/buyer: pending. What failed or changed, and why: pending.

Gate and capacity

Gate requirement: No standalone gate; check this week's acceptance criteria and next gate dependencies. Gate status: NOT_STARTED. Original target week: See next gate in specs. Actual completion date / forecast: not set. Missing external evidence: pending inspection. Blocks completed / 7: not recorded. Days with commits / 7: not recorded. Sunday build log / optional Wednesday note: not recorded. Founder conversations cumulative: not recorded. Earned evidence level and proof: not assessed. Consecutive lost weeks: not assessed.

Week-specific risk to revisit: Do not force wet-lab results to arrive on schedule. Two major workstreams compete for capacity; prioritize contracted obligations and record research slippage rather than inventing closure.

Decision and next-model handoff

Continue / recover / blocked: not decided. Retain as the minimum: Complete the contracted audit readout or documented delivery recovery, and preserve any received partner data with provenance. Skipped permanently with reason: none recorded. Carryover with owner, dependency and target week: none recorded. Canonical repository paths and versions: pending. Data permissions, split definitions or assumptions to preserve: pending. External action authorization that still applies: not assessed. Next physical action, exact file/function/paragraph: pending. Updates to root PROGRESS / LOG / TODAY completed: ☐

Next week

✎

YOUR SPACE

Notes, links & media

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    ⏱ Focus Focus25:00 0 min today