scanpy "Preprocessing and clustering 3k PBMCs" tutorial
scanpy "Preprocessing and clustering 3k PBMCs" tutorial (scanpy.readthedocs.io, tutorials section) (75 min, run it)
In: LABLOOP-W19 Notebook
WEEK 19 · Mon 25 Jan → Sun 31 Jan 2027
UNDERSTAND · PRACTICE · KEEP EVIDENCE
Read the week in plain language, refresh the foundations, follow each challenge step by step, and check what evidence to keep. Includes worked examples, answer keys and prompts for visual study resources.
Use this within your existing weekly blocks. Your learning completion and research evidence remain in your current tracker and week files.
START HERE · 2 MINUTES
STATUS
| Deliverable | Acceptance | State |
|---|---|---|
paper-2-venue-decision.mdBuild create + |
Confirm endorsement eligibility/status or prepare a scope-checked fallback submission route. Acceptance: A dated route and current requirements are recorded without implying endorsement. | |
paper-2-review-response.mdBuild create + |
Resolve model and human reviews, audit intervals and prepare a five-minute technical defense of Paper 2. Acceptance: Essential scientific concerns are closed or submission remains blocked. | |
career-launch-pack.mdBuild create + |
Prepare a one-screen portfolio, ATS-readable CV, short scientific bio, recruiter message and five tailored application packages. Acceptance: Each skill claim links to proof; Paper 2 is labeled draft until public. | |
study-notes.mdLearning create + |
Selected exact free resources; bounded lesson/exercise; separate solution; quiz and grading rubric; Peter's answers and gaps when executed | |
founder-paper.mdFounder papers create + |
Verified source and P11 digest; authentic question and connection sentence; access limitations | |
branding-pack.mdBranding create + |
Wednesday note, Sunday build log, X adaptation, figure/caption/alt text and the week's special asset; each claim linked to real proof | |
opportunity-actions.mdOpportunities create + |
Qualified target/role, source/date, ladder stage, next action, message/interview/proposal draft and actual status | |
evidence.mdEvidence & review open & edit ↗ |
Actual paths/URLs, versions, checks/metrics, preparation versus execution state and external receipts when applicable | |
review.mdEvidence & review open & edit ↗ |
Checklist, learning assessment, gate status, shortfalls, next actions and carryover |
These filenames are outputs to prepare during this week's execution; this scaffold does not claim they already exist. Drafts and measured results have different states. Technical code/data stay in the canonical project repository and are referenced from these coordinating files.
During preparation, future measured tables get column definitions, input requirements and run instructions with PENDING status. During execution, replace pending values only with measured outputs. Do not create realistic-looking fake evidence.
600 MINUTES MAXIMUM
Target. Confirm endorsement eligibility/status or prepare a scope-checked fallback submission route. Stop when: A dated route and current requirements are recorded without implying endorsement.
No material for Monday — this is a pure build day. Need something to study instead? →
Target. scanpy PBMC 3k tutorial — scanpy.readthedocs.io Produce: Notebook run end to end
Do these in this order — the plan's own sequence:
Target. Resolve model and human reviews, audit intervals and prepare a five-minute technical defense of Paper 2. Stop when: Essential scientific concerns are closed or submission remains blocked. Reserve the last 10 minutes for the optional Wednesday note.
No material for Wednesday — this is a pure build day. Need something to study instead? →
Target. MSMB ch. on reporting; bootstrap intervals (ISLP ch. 5.2) Select material/exercise that fits 60 minutes; use the last 30 for the founder paper.
Do these in this order — the plan's own sequence:
Target. Prepare a one-screen portfolio, ATS-readable CV, short scientific bio, recruiter message and five tailored application packages. Stop when: Each skill claim links to proof; Paper 2 is labeled draft until public.
No material for Friday — this is a pure build day. Need something to study instead? →
Target. Resolve the earliest critical spillover or dependency. Prepare any long-form publication draft from existing figures here if needed. Sandbox only after commitments are met.
Optional, only if the day went well:
Target. 20 min build-log draft/publication when authorized; 5 min evidence/checklist; 20 min source-grounded gap review; 15 min next week's six physical actions.
No material for Sunday — this is a pure build day. Need something to study instead? →
Spare pockets, repo exercises and anything the plan lists without a day. Take them in this order when a day finishes early.
The model must turn these into exact physical actions using the observed files and available inputs. Each 90-minute block includes start/stop rituals. All calls, posts, debugging, reading and exercises consume this same capacity.
The source lists more material than necessarily fits. Select exact excerpts for the learning objective and park the remainder; no full-course completion requirement.
WHAT TO WATCH, READ AND RUN
Around this point in an expedition, the team walks into a town for the first time in months. People want to know who they are. You need three things: a letter of introduction (the endorser's yes), a second pair of eyes on the report (review), and a sign on the door (a one-screen site). You also start knocking on doors that were closed at week 0: five applications to scientific-data and bioprocess-AI companies with y…
0 of 10 finished · give me one to start now → · open in the deck →
In the plan's running order: each card says which block it belongs to and what has to come before it. The seven-day plan lists the same cards under the day that owns them.
scanpy "Preprocessing and clustering 3k PBMCs" tutorial (scanpy.readthedocs.io, tutorials section) (75 min, run it)
In: LABLOOP-W19 Notebook
Modern Statistics for Modern Biology sections on reporting and ISLP ch. 5.2 (bootstrap) re-read (40 min); apply bootstrap intervals across both papers
In: LABLOOP-W19 Notebook
Saturday sandbox
Single-cell best practices (Heumos et al., theislab) — sc-best-practices.org, introduction and quality-control chapters
In: LABLOOP-W19 Notebook
Saturday sandbox · after Single-cell best practices
MIT 6.874 single-cell lecture (Kellis, YouTube)
In: LABLOOP-W19 Notebook
Saturday sandbox · after MIT 6.874 single-cell lecture
Cumming (2014), "The new statistics: why and how", Psychological Science 25(1):7-29 — the case for estimation and intervals over p-values
In: LABLOOP-W19 Notebook
Saturday sandbox · after Cumming (2014), "The new statistics: why and how"
Chip Huyen, ML Interviews Book — huyenchip.com/ml-interviews-book (free) — chapters on the interview process, for the applications you send this week
In: LABLOOP-W19 Notebook
StatQuest "PCA main ideas", "t-SNE, Clearly Explained" and "UMAP Dimension Reduction, Main Ideas!!!" (YouTube, 30 min) — the three plots every single-cell talk shows
In: LABLOOP-W19 Notebook
PBMC 3k tutorial notebook (in the docs; scverse/scanpy-tutorials is archived, ★ 242) in Colab
In: LABLOOP-W19 Notebook
Run the quality-control notebook on the same data; compare cell counts after filtering
In: LABLOOP-W19 Notebook
scipy.stats.bootstrap on your key metrics; replace every naked number in both papers
In: LABLOOP-W19 Notebook
READ FIRST
This week delivers the three concrete artifacts below and the supporting learning/branding/opportunity pack. The scientific or commercial outcome follows the preserved source brief, while the acceptance checks make its evidence testable. The career objective is to turn this work into something a scientist, hiring manager or buyer can inspect.
READ FIRST
Read v6 · Week 19 · Endorsement closed; review; site before Monday. It carries this week's v5 brief unchanged and adds the Welcome analogy, Learn it (▶ core and + deeper sources), Run it (repos with a 20-40 minute exercise), the NotebookLM kit and the mentor check below. Standing v6 rules: resource rules, NotebookLM protocol, chapter anatomy. v6 adds depth only; it does not change a goal, gate, output or the 600-minute cap, so this specification still governs execution.
Act IV · Threads: B, M, BL, R · Next gate: 20
Where this week sits. - ← From: week 12 endorsement routes, week 15 reviewer, weeks 16-18 preprints and drafts. - → Feeds: week 20 (submission), week 27 (offer page links to the site), week 32 (second round of applications), week 37 (interviews). - ⟳ Returns in: every gate, when the site and headline update.
Goals. - 🎯 This week: endorser confirmed (or bioRxiv fallback ready); Paper 2 reviewed twice by P10 + one human; one-screen site live; 5 applications sent; scanpy tutorial run end to end. - 🏁 Next milestone: Gate 20. - 🌟 Final: a public identity that matches the work.
Mentor check. "Someone who never met me can open one page and see two preprints, one instrument, one dataset and how to reach me."
OPEN WHEN NEEDED
READ FIRST
Source unit: B19 · Omics literacy
Material: scanpy PBMC 3k tutorial — scanpy.readthedocs.io
Produce: Notebook run end to end
Understanding check: You can follow a single-cell talk
During preparation, write one worked example connected to the week's actual task, one exercise with a separate answer key and five questions. Peter supplies answers; score explanation quality and flag misconceptions in QUESTIONS. Numeric/scientific conclusions require actual data or explicitly synthetic teaching values.
Source unit: M19 · Effect sizes and honest reporting
Material: MSMB ch. on reporting; bootstrap intervals (ISLP ch. 5.2)
Produce: Audit uncertainty for each estimated performance measure and effect in Papers 1–2; raw counts, identifiers and fixed configuration values do not require artificial intervals.
Understanding check: Peter can justify the uncertainty method and independent sampling unit for each major estimate.
Use the prompt pack: P1 explanation ladder, P2 concept-to-project bridge and P3 quiz. Coding practice belongs within the allocated study/build block. Where the source asks for a favorable scientific result, substitute an honest measured comparison as explained below.
Select one verified founder/scientific-lead paper relevant to this week's domain. Use P11; save the question, method, numerical result with context, data workflow, three author questions and one connection sentence in founder-paper.md. Keep abstract-only access limitations explicit. On source weeks with no study, prioritize the commercial/submission task and log any skipped digest.
OPEN WHEN NEEDED
Branding: Portfolio tells one story: leadership experience plus measured scientific work; feature dataset, instrument and manuscript status.
Opportunity action: Verify five current employer openings or speculative routes; rank technical fit, geography, language, seniority and v4 culture filter.
Use career asset contracts. Publish at most the agreed cadence; one figure and one supported number or admitted failure can support multiple drafts. In long-form weeks, prepare a 1,500–2,500-word article from existing evidence within the build/buffer allocation. In release/demo weeks, prepare a 60-second script: problem, input, command/action, result, limitation and proof link.
Verify individuals, jobs and affiliations at execution time. The GridX list and v4 company map are research seeds, not current facts. Do not reveal named company weaknesses or employer/client confidential information. Keep drafted/sent/replied/booked/held statuses separate. The planning request alone does not authorize external messages or publication; honor explicit authorization in the execution session.
OPEN WHEN NEEDED
v5 asks for two preprints in week 19 although Paper 2 is scheduled for week 20. Use the available draft honestly; current vacancies and company status must be checked at execution.
Apply the root scientific evidence contract: provenance; meaningful independent splits; no future-data leakage; baseline comparison; actual denominators/uncertainty; failed cases; human review where required. State whether data are simulated, public experimental, synthetic or partner-provided.
Keep source gates intact, and log scope/time conflicts in review. Missing people, money, access or results must not be replaced with invented evidence. Reduce optional polish first. A specific essential external dependency stays pending while independent preparation continues.
READ FIRST
This is an enabling week. Its deliverables feed the next scheduled gate: 20. Complete the acceptance checklist below; do not promote reputation from a draft.
READ FIRST
Source-preserving minimum: the endorser's yes.
Operational first step if stalled: open paper-2-venue-decision.md, write the required inputs and produce the smallest checkable example in ten minutes. If a more urgent missing gate dependency controls the week, use the source minimum above.
If the full week does not fit, use P14: retain one gate-critical deliverable and one directly supporting study exercise; record what is skipped versus moved. Do not mark the original full gate passed merely because the minimum was completed. After a missed week, reforecast from actual evidence and avoid a second unexamined lost week.
OPEN WHEN NEEDED
In review, state exactly what exists, what was verified, what Peter can now explain, which external events remain pending, where the canonical code/data live, and the next file/function or draft paragraph to touch. Link each fact to evidence. Carry scientific assumptions, split definitions and rights constraints forward explicitly.
OPEN WHEN NEEDED
Prepare Week 19 of this LABLOOP execution plan.
Read execution-plan/specs.md, execution-plan/DECISIONS.md,
execution-plan/GATES.md and
execution-plan/weeks/week-19-recruiter-portfolio-and-review/specs.md, commitment.md, evidence.md and review.md.
Read prior prerequisite reviews and inspect actual repository/data state.
Use v5 as the governing sequence, the v6 Week 19 chapter
(LABLOOP-Master-Plan-v6.md) as the deeper walkthrough, and v4 only for
retained details.
Produce the full local preparation pack and the exact week-specific files named
in the artifact table. Give me a bounded lesson with exercises/answer key,
daily physical actions, technical runbook or draft, branding drafts and
career/commercial actions. Keep the total to 600 minutes.
Separate prepared artifacts from measured results and my learning completion.
Verify sources and do not invent metrics, citations, papers, people or outcomes.
Do not execute external communications, publication or spending unless the
current session already authorizes the specific action.
Finish by updating evidence/review and TODAY with the next physical action.
OPEN WHEN NEEDED
v6 reproduces this brief unchanged inside v6 · Week 19 · Endorsement closed; review; site. The following is source planning text, not a claim of completion. Apply the explicit evidence corrections in this specification and DECISIONS.md.
WEEK FILES
State: UNCONFIRMED. Owner: Peter Ciancaglini. Specification
Start date: not set. End date: not set. Available hours: confirm (planned ceiling 10). Primary outcome: recruiter portfolio and review. Minimum that keeps the next gate alive: the endorser's yes.
paper-2-venue-decision.md: A dated route and current requirements are recorded without implying endorsement.paper-2-review-response.md: Essential scientific concerns are closed or submission remains blocked.career-launch-pack.md: Each skill claim links to proof; Paper 2 is labeled draft until public.| Day | Minutes | Physical action / outcome | Actual slot | Done |
|---|---|---|---|---|
| Mon | 90 | Confirm endorsement eligibility/status or prepare a scope-checked fallback submission route. | Not set | ☐ |
| Tue | 90 | Notebook run end to end | Not set | ☐ |
| Wed | 90 | Resolve model and human reviews, audit intervals and prepare a five-minute technical defense of Paper 2. Last ten minutes: optional note. | Not set | ☐ |
| Thu | 90 | Every number in Papers 1-2 has an interval Include relevant founder-paper work within this block. | Not set | ☐ |
| Fri | 90 | Prepare a one-screen portfolio, ATS-readable CV, short scientific bio, recruiter message and five tailored application packages. | Not set | ☐ |
| Sat | 90 | Resolve critical spillover; long-form draft if due; sandbox only if done. | Not set | ☐ |
| Sun | 60 | Build log, evidence, gap review, next actions. | Not set | ☐ |
Peter's commitment / adjustments: pending. Reviewing model and date: pending.
Execution state: NOT_STARTED. Specification This file is a blank register with planned artifact names; none of the rows prove completion.
| Item | Path / URL / version | State | Verification / actual result | Verified by / date |
|---|---|---|---|---|
| paper-2-venue-decision.md | Not created | NOT_STARTED | Pending: A dated route and current requirements are recorded without implying endorsement. | — |
| paper-2-review-response.md | Not created | NOT_STARTED | Pending: Essential scientific concerns are closed or submission remains blocked. | — |
| career-launch-pack.md | Not created | NOT_STARTED | Pending: Each skill claim links to proof; Paper 2 is labeled draft until public. | — |
| Learning exercise / Peter's quiz | Not created | NOT_STARTED | Peter's actual answers required | — |
| Founder paper digest | Not created | NOT_STARTED | Source/content and access checked | — |
| Branding pack | Not created | NOT_STARTED | Claims audited; drafted/public status distinct | — |
| Opportunity actions | Not created | NOT_STARTED | Drafted/sent/replied/booked/held distinct | — |
| Question / metric | Dataset, N and unit | Split / seed / config | Command or verification method | Output path and measured value | Uncertainty / limitation |
|---|---|---|---|---|---|
| Date | Action / counterpart | Receipt or private reference | State | Next action / date |
|---|---|---|---|---|
Peter's teach-back / quiz score: not assessed. Scientific assumptions and deviations: none recorded yet. Budget allocated / spent: not assessed / no spend recorded. Missing evidence and responsible owner: pending inspection.
Do not put API keys, source full texts with restricted rights, client data or invoice personal details in a public version of this register. Keep private evidence references private.
Review status: NOT_REVIEWED. Specification · Evidence
What Peter can explain or reproduce now: not assessed. Quiz / teach-back gaps: not assessed. Measured result and limitations: pending. Best evidence link for a scientist/recruiter/buyer: pending. What failed or changed, and why: pending.
Gate requirement: No standalone gate; check this week's acceptance criteria and next gate dependencies. Gate status: NOT_STARTED. Original target week: See next gate in specs. Actual completion date / forecast: not set. Missing external evidence: pending inspection. Blocks completed / 7: not recorded. Days with commits / 7: not recorded. Sunday build log / optional Wednesday note: not recorded. Founder conversations cumulative: not recorded. Earned evidence level and proof: not assessed. Consecutive lost weeks: not assessed.
Week-specific risk to revisit: v5 asks for two preprints in week 19 although Paper 2 is scheduled for week 20. Use the available draft honestly; current vacancies and company status must be checked at execution.
Continue / recover / blocked: not decided. Retain as the minimum: the endorser's yes. Skipped permanently with reason: none recorded. Carryover with owner, dependency and target week: none recorded. Canonical repository paths and versions: pending. Data permissions, split definitions or assumptions to preserve: pending. External action authorization that still applies: not assessed. Next physical action, exact file/function/paragraph: pending. Updates to root PROGRESS / LOG / TODAY completed: ☐
YOUR SPACE